Skincare routines have long relied on the classic cleanse-tone-moisturize trio. For many, it's even ballooned into a 10-step routine full of serums and under-eye masks. We've all followed these steps, but have you ever wondered if using these products together benefits your skin?Science shows that effective skin care goes beyond mixing and matching products from various brands. It's about synergy. Thatβs why the OneSkin Longevity Protocol is meticulously formulated and scientifically validated to work in harmony, battling aging at the cellular level from head to toe.Still skeptical? Let us walk you through the science behind our unique protocol.
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Step 01: Cleanse And Exfoliate With PREP
When we set out to create our daily cleanser, we aimed for more than just removing impurities. Thatβs why PREP is formulated with gentle yet effective chemical exfoliators like prickly pear extract; these ingredients not only clear away dead skin cells but also enhance your skin's absorption of subsequent products. PREP was rigorously tested in the OneSkin lab to confirm its ability to boost the effects of OS-01 FACE. The results were impressive.
PREP Doubles The Absorption of The OS-01 PeptideΒ
The OS-01 peptide works at the cellular level to target a root cause of skin aging, boosting epidermal thickness, collagen and hyaluronic acid production, and cellular repair. (1,2) In order for the OS-01 peptide to work at the cellular level, it needs to reach those cells deep within skin. The The OneSkin Lab found cleansing with PREP before using OS-01 FACE increases the absorption of the peptide by 2.2X, compared to using OS-01 FACE alone. (1)This means that using PREP prior to OS-01 FACE could more than double the amount of peptide in the dermis layer of skin. This means that your collagen-producing fibroblast cells will receive more peptide, and you should experience enhanced effects from using OS-01 FACE by cleansing with PREP prior.
Shown by comparing the amount of the OS-01 peptide present in the dermis layer of ex vivo human skin samples exposed to OS-01 FACE and pre-cleansed with PREP versus not pre-cleansed at all.
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Step 02: Use OS-01 EYE To Slow Aging Near The Eyes
Considering that the skin around your eyes ages faster than the skin on the rest of your face, it's a shame that most skin care protocols don't include a dedicated eye cream. (3) To combat accelerated aging around the eyes, we created our most potent product - OS-01 EYE.Formulated with the highest concentration of the OS-01 peptide and other star ingredients like Argirilene and CoQ10, OS-01 EYE specifically addresses the needs of the eye area.Read more about how our OS-01 EYE is distinct from OS-01 FACE here.
OS-01 EYE Doubles Collagen In The Dermal Layer
By measuring collagen levels in ex vivo human eyelid samples, we found that OS-01 EYE improves collagen levels by 2.3x in the dermis.(1)By improving collagen in this structural skin layer, OS-01 EYE helps the thin skin around the eyes resist accelerated age-related collagen loss, leading to firmer, more radiant, and elastic skin.(1)
Shown in ex vivo human eyelid skin samples from a 70 yo male donor, by measuring collagen levels in the dermis before (Control) and after treatment with OS-01 EYE. Data measured in triplicate and normalized to control. The collagen density was determined by quantifying Masson's Trichrome staining.
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By improving skin health at the cellular level, OS-01 EYE results in visible skin changes around the eyes, including:
- Improved skin elasticity (Shown in 86% of users)*
- Improved hydration (Avg improvement of +32%)*
- Skin barrier (Avg improvement of +17%)*
- Improved overall appearance (Shown in 96% of users)*
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Step 03: Use OS-01 FACE To Support Epidermal Thickness
At the heart of any complete skincare protocol is a quality moisturizer. However, Oneskins' flagship product, OS-01 FACE, is so much more than that.
OS-01 FACE is formulated to address the root causes of aging in the most vulnerable and exposed areas of your skin, like the face, neck, and hands.
OS-01 FACE Increases Epidermal Thickness and Barrier Function
Epidermal thickness plays a crucial role in maintaining the youthful appearance and overall health of your skin and is a key factor in barrier function. Skin naturally thins with age, leading to a loss of elasticity, increased fragility, and less protection for your body from the outside world, making epidermal thickness an essential element of skin longevity and whole-body health. OS-01 FACE is scientifically proven to increase epidermal thickness, translating to smoother, more youthful-looking skin and a clinically validated increase in barrier function. (1,2,6)
Epidermal thickness analysis of ex vivo skin samples (35, 55 and 79 yo) maintained in basal media, or treated with OS-01 FACE for 5 days. (Zonari, et al., 2023, npj Aging, 2023)
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By improving collagen & epidermal thickness in skin thatβs vulnerable to damage, OS-01 FACE was clinically validated to provide visible improvements, including:(1,6)
- Improved skin smoothness & overall appearance (Shown in 100% of users)*
- Improved skin evenness, radiance, pores, & firmness (shown in 95.5% of users)*
- Improved skin elasticity (Shown in 90% of users)*
- Diminished appearance of fine lines & wrinkles (Shown in 87% of users)*
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Step 04: OS-01 FACE SPF Undoes the Effects of UV Aging
While OS-01 FACE is an unprecedented solution to intrinsic skin aging, sunscreen is still the most effective way to prevent future extrinsic aging from UV. After all, long-term damage from UV exposure can trigger up to 80% of the visible signs of aging.[8] Thatβs why OneSkin offers OS-01 FACE SPF - a 100% non-nano mineral sunscreen specifically designed for delicate facial skin and available in untinted and sheer tinted shades.
Using OS-01 FACE SPF and OS-01 FACE Enhanced the Longevity Boosting Effects of the OS-01 Peptide
Not only does OS-01 FACE SPF contain non-nano zinc oxide to prevent UV-induced skin damage, it also contains the OS-01 peptide to target existing aging; however, the penetration of the peptide is naturally inhibited by zinc oxide, our preferred UV filter. Therefore, to reap the full benefit of the OS-01 peptide, we recommend using OS-01 FACE as your daily facial moisturizer and OS-01 FACE SPF as your daily facial SPF. In fact, using OS-01 FACE SPF & OS-01 FACE together enhances the effects of the OS-01 peptide.When used in conjunction with OS-01 FACE, OS-01 FACE SPF significantly reduces the expression of key biomarkers associated with aging. (1)
The expression of biomarkers associated with aging, P16 and IL-8, were measured on ex vivo human skin samples before treatment and after treatment with OS-01 FACE alone, or OS-01 FACE then OS-01 FACE SPF (formerly OS-01 SHIELD).
This enhanced effect of using both OS-01 FACE and OS-01 FACE SPF together is likely due to the increased activity of the OS-01 peptide.
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Step 05: OS-01 BODY Helps Boost Barrier Function
Most skin care protocols focus on the skin on your face. But about 97% of your skin is below your neck! Considering that skin is your bodyβs primary defense from the outside world, and its deterioration is linked to age-related diseases, Oneskinβs protocol includes OS-01 BODY, designed to fortify your largest vital organ.[9] In an unprecedented clinical study, Oneskins scientists were delighted to find that OS-01 BODY successfully paused overall biological aging and reduced a key inflammation biomarker in the blood, pointing to the important role that the skin barrier plays in overall inflammation and longevity (1)
OS-01 BODY Increases Epidermal Thickness
As your skin ages, the epidermis begins to thin. One study found that it thins at a rate of about 5-7% per decade. (10) This can lead to common signs of aging on the body, including crepiness and skin laxity, leave your body vulnerable with less protection from external aggressors, and increase rates of inflammation (aka inflammaging).[11] To guarantee that OS-01 BODY improved these common signs of aging, Oneskin scientists analyzed whether it could increase epidermal thickness in the lab.Compared to skin that was not treated, ex vivo human skin samples treated with OS-01 BODY showed up to a 40% increase in epidermal thickness and increased barrier function. (1) This improved skin thickness may lead to a reduced appearance of crepey skin, an improved feeling of skin hydration, and even a reduction in inflammatory cytokines.
Epidermal thickness analysis of ex vivo skin sample from a 44 year old donor measured before and after treatment with OS-01 BODY. Data measured in triplicate and normalized to control.
In fact, in a 12-week clinical study, OS-01 BODY was found to (1):
- Increase skin barrier function by +41.49% on the forearm
- Improve hydration in 93% of users
- Decrease the level of circulating IL-8 significantly
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Step 06: OS-01 BODY SPF Protects Against UV Aging
Before you step into the sun, the skin on your body still faces the most significant source of extrinsic aging: UV radiation. OS-01 BODY SPF is a crucial part of Oneskinβs longevity protocol, specifically for the skin on your body that is exposed to the sun to reduce both intrinsic and extrinsic sources of aging.Β
OS-01 BODY SPF Reduces The Effects of UV Aging
To understand how OS-01 BODY SPF mitigates skin aging, Oneskin scientists analyzed ex vivo human skin samples treated with the product versus a control after UVB exposure. Results showed OS-01 BODY SPF reduced key aging biomarkers p16 and IL-8. (1)OS-01 BODY SPF also mitigated hyaluronic acid and collagen degradation by lowering HYAL1 and MMP-1 levels. (1) We believe that OS-01 BODY SPF effectively reduces the effects of UV exposure on skin by targeting multiple aging pathways, including UV damage, oxidative damage, and senescence with non-nano zinc oxide, a blend of potent antioxidants, and the OS-01 peptide, respectively.
Ex vivo human skin samples (41y and 59y) that were treated with nothing (No Treatment) or OS-01 BODY SPF were exposed to 1J/cm2 UVB radiation, then the gene expression levels were analyzed after 24 hours.
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How to Use Oneskinβs Complete Protocol Together
While the OneSkin protocol contains everything you need, some people prefer to integrate it with their existing routines. Hereβs your guide for getting the application order just right.
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Morning:
- Use PREP to cleanse, or skip it if your face is still clean from the night before
- Apply OS-01 EYE by gently tapping one pump in a semi-circle around each eye
- Follow with OS-01 FACE. Allow both OS-01 EYE and OS-01 FACE to absorb
- Apply OS-01 FACE SPF and OS-01 BODY SPF to exposed areas as the last step in your skin care routine
- You can follow up with your choice of makeup
Evening:
- Cleanse with PREP to remove your SPF and makeup
- Double cleanse (optional)
- Apply OS-01 EYE
- Follow with OS-01 FACE
- If you've worn OS-01 BODY SPF, shower before applying OS-01 BODY to clean body skin (damp or dry) and massage until absorbed
Evening:
- Cleanse with PREP to remove your SPF and makeup
- Double cleanse (optional)
- Apply OS-01 EYE
- Follow with OS-01 FACE
- If you've worn OS-01 BODY SPF, shower before applying OS-01 BODY to clean body skin (damp or dry) and massage until absorbed
Key Takeaways:
- Unlike many skin care protocols, OneSkin products were specifically formulated to work better together.
- Using PREP before OS-01 FACE has been scientifically proven to double the penetration of the OS-01 peptide, so you get maximum benefits from every pump.
- At the heart of our protocol is OS-01 FACE, the daily moisturizer proven to improve skin barrier function
- Plus, our protocol contains a body cream to support longevity in the 97% of skin not on your face or neck, fortifying your bodyβs largest organ.
- OS-01 FACE SPF and OS-01 BODY SPF are formulated to target multiple aging pathways with non-nano zinc oxide, potent antioxidants, and the OS-01 peptide
Disclaimers
- This article was initially published in January 2023 and has been updated.
References
- Based on data from clinical studies and/or lab studies conducted on human skin samples, 3D skin models, and skin cells in the OneSkin lab. Explore more at oneskin.co/claims
- https://www.nature.com/articles/s41514-023-00109-1
- https://www.jaad.org/article/S0190-9622(16)00242-5/fulltext
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268288/
- https://doi.org/10.1016/j.jaad.2016.02.112
- https://onlinelibrary.wiley.com/doi/epdf/10.1111/jocd.16242
- https://pubmed.ncbi.nlm.nih.gov/10048311/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344124/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697145/
- https://pubmed.ncbi.nlm.nih.gov/2384222/
- https://www.aging-us.com/article/102946/text
