How OS-01 Works on Mature Skin

5 min read

MARCH 23, 2022
ONESKIN PRODUCTS

How OS-01 Works on Mature Skin

5 min read

MARCH 23, 2022
ONESKIN PRODUCTS
When we’re young, building a skin care routine is relatively simple: a face wash, moisturizer and sunscreen will do the trick. But as we grow older and develop visible signs of aging skin, choosing from the overwhelming array of mature skin care products like anti-aging creams, serums, and treatments often feels like an impossible task. What can we trust to actually work?
When we’re young, building a skin care routine is relatively simple: a face wash, moisturizer and sunscreen will do the trick. But as we grow older and develop visible signs of aging skin, choosing from the overwhelming array of mature skin care products like anti-aging creams, serums, and treatments often feels like an impossible task. What can we trust to actually work?

It’s easy to get lost in new trends like 10-step skincare routines, but try to look past marketing and prioritize your skin’s health above all else. As our largest organ and first line of defense against environmental aggressors, our skin can have an enormous impact on our overall body health. A truly effective product should support your skin’s health first and improve the way it looks as a result. That’s where OneSkin comes in.Our Topical Supplements are powered by OS-01: the first peptide scientifically proven to reduce skin’s biological age at the molecular level. [1] So skin doesn’t just look younger, it actually feels like younger skin. To understand how our OS-01 topical supplements are different from standard anti-aging products, let’s take a closer look at why skin ages in the first place.

It’s easy to get lost in new trends like 10-step skincare routines, but try to look past marketing and prioritize your skin’s health above all else. As our largest organ and first line of defense against environmental aggressors, our skin can have an enormous impact on our overall body health. A truly effective product should support your skin’s health first and improve the way it looks as a result. That’s where OneSkin comes in.Our Topical Supplements are powered by OS-01: the first peptide scientifically proven to reduce skin’s biological age at the molecular level. [1] So skin doesn’t just look younger, it actually feels like younger skin. To understand how our OS-01 topical supplements are different from standard anti-aging products, let’s take a closer look at why skin ages in the first place.
01

Understanding Biological Skin Aging

If you’ve ever had a doctor tell you that you have the “heart of a 20 year old”, you’re already familiar with the concept of biological age. No matter how many candles are on your birthday cake, your body might function like an older or younger body based on your health status.Unlike chronological age, biological age is malleable and can be increased or decreased based on your daily habits. For example, if you regularly wear SPF, your skin is likely biologically younger than it would be if you experienced a lot of unprotected sun damage.So how does the OS-01 peptide reduce our skin’s biological age? By targeting a main driver of aging: cellular senescence. [1] Read on to find out just how much the OS-01 peptide impacts cellular senescence and what that means for its effects on mature and aging skin.
01

Understanding Biological Skin Aging

If you’ve ever had a doctor tell you that you have the “heart of a 20 year old”, you’re already familiar with the concept of biological age. No matter how many candles are on your birthday cake, your body might function like an older or younger body based on your health status.Unlike chronological age, biological age is malleable and can be increased or decreased based on your daily habits. For example, if you regularly wear SPF, your skin is likely biologically younger than it would be if you experienced a lot of unprotected sun damage.So how does the OS-01 peptide reduce our skin’s biological age? By targeting a main driver of aging: cellular senescence. [1] Read on to find out just how much the OS-01 peptide impacts cellular senescence and what that means for its effects on mature and aging skin.
02

How OS-01 Works: Reducing Senescent Burden in Skin

Cellular senescence is the end stage in a cell’s life cycle when it stops dividing. When we’re young and healthy, senescent skin cells are quickly cleared away and replaced with new cells. But as we get older, our body becomes less efficient at clearing away senescent cells. Like rotten apples that spoil the bunch, senescent cells drive healthy neighboring cells into senescence, triggering widespread damage that can make our skin look and function like older skin.By reducing the number of senescent cells in the skin, senomorphic compounds like our proprietary OS-01 peptide have the potential to slow down and even reverse biological skin aging. [1]Just how much can the OS-01 peptide reduce senescent burden in the skin? Our scientists ran an experiment to answer that exact question. Using beta-galactosidase, an enzyme that selectively stains senescent cells blue, we found that OS-01 reduced senescent cells by up to 50% in in vitro fibroblast cultures from patient derived samples. [1]
02

How OS-01 Works: Reducing Senescent Burden in Skin

Cellular senescence is the end stage in a cell’s life cycle when it stops dividing. When we’re young and healthy, senescent skin cells are quickly cleared away and replaced with new cells. But as we get older, our body becomes less efficient at clearing away senescent cells. Like rotten apples that spoil the bunch, senescent cells drive healthy neighboring cells into senescence, triggering widespread damage that can make our skin look and function like older skin.By reducing the number of senescent cells in the skin, senomorphic compounds like our proprietary OS-01 peptide have the potential to slow down and even reverse biological skin aging. [1]Just how much can the OS-01 peptide reduce senescent burden in the skin? Our scientists ran an experiment to answer that exact question. Using beta-galactosidase, an enzyme that selectively stains senescent cells blue, we found that OS-01 reduced senescent cells by up to 50% in in vitro fibroblast cultures from patient derived samples. [1]
The OS-01 peptide also maintained the total number of cells, meaning it promoted not just the reduction of senescent cells, but also the renewal of healthier ones.
The OS-01 peptide also maintained the total number of cells, meaning it promoted not just the reduction of senescent cells, but also the renewal of healthier ones.
03

What OS-01 Does For Mature Skin?

Now that we know how the OS-01 peptide works, let’s talk about what the OS-01 peptide actually does for mature skin.To analyze OS-01’s effects on the skin, our R&D team utilized skin cells from a variety of donors of different ages, ethnicities, and skin types to grow human skin models, along with human skin samples, then exposed them to the peptide. By first exposing these skin models to the OS-01 peptide, then utilizing quantitative and qualitative analysis techniques, our researchers measured the effects of OS-01.In tests, the OS-01 peptide reliably and effectively improved markers of skin health and aging including: [1]
  • Improving cellular structure within the skin
  • Increasing skin’s epidermal thickness
  • Reducing skin’s biological age, based on DNA markers
Let’s take a deeper look at these effects.
03

What OS-01 Does For Mature Skin?

Now that we know how the OS-01 peptide works, let’s talk about what the OS-01 peptide actually does for mature skin.To analyze OS-01’s effects on the skin, our R&D team utilized skin cells from a variety of donors of different ages, ethnicities, and skin types to grow human skin models, along with human skin samples, then exposed them to the peptide. By first exposing these skin models to the OS-01 peptide, then utilizing quantitative and qualitative analysis techniques, our researchers measured the effects of OS-01.In tests, the OS-01 peptide reliably and effectively improved markers of skin health and aging including: [1]
  • Improving cellular structure within the skin
  • Increasing skin’s epidermal thickness
  • Reducing skin’s biological age, based on DNA markers
Let’s take a deeper look at these effects.
04

OS-01 Increases Epidermal Thickness

If you feel like your skin is getting more delicate with time, you’re not alone. As we age, our epidermal layer thins – resulting in telltale signs of aging like wrinkles, crepiness, and skin fragility. This thinning impacts not just the skin’s appearance, but also the body’s overall health. As the epidermis becomes thinner, the skin becomes more susceptible to tearing and injury–leaving the body more vulnerable to external pathogens.
04

OS-01 Increases Epidermal Thickness

If you feel like your skin is getting more delicate with time, you’re not alone. As we age, our epidermal layer thins – resulting in telltale signs of aging like wrinkles, crepiness, and skin fragility. This thinning impacts not just the skin’s appearance, but also the body’s overall health. As the epidermis becomes thinner, the skin becomes more susceptible to tearing and injury–leaving the body more vulnerable to external pathogens.
Histology images of ex vivo human skin samples before and after treatment with the OS-01 peptide. [1]
Histology images of ex vivo human skin samples before and after treatment with the OS-01 peptide. [1]

The OS-01 peptide can promote epidermal thickness to help keep your skin stronger, firmer, and smoother. In the lab, this impact is obvious just by examining the skin structure under a microscope before and after treatment with the OS-01 peptide. By comparing these skin histology images, you can see that after exposure to OS-01:
  • Epidermal thickness increased by 28%
  • Cellular structure appears more defined
  • Connectivity appears to be improved
These findings are especially important for mature skin concerns. By thickening the epidermal layer, the OS-01 peptide can strengthen the skin barrier [1] – helping the skin resist injuries that can occur later in life.

The OS-01 peptide can promote epidermal thickness to help keep your skin stronger, firmer, and smoother. In the lab, this impact is obvious just by examining the skin structure under a microscope before and after treatment with the OS-01 peptide. By comparing these skin histology images, you can see that after exposure to OS-01:
  • Epidermal thickness increased by 28%
  • Cellular structure appears more defined
  • Connectivity appears to be improved
These findings are especially important for mature skin concerns. By thickening the epidermal layer, the OS-01 peptide can strengthen the skin barrier [1] – helping the skin resist injuries that can occur later in life.
05

OS-01 Reduces Skin’s Biological Age

With time, our DNA actually changes: accumulating telltale markers of aging. No matter how young your skin looks, these DNA modifications predict whether your skin is functioning like younger, healthier skin or like older, more vulnerable skin.To understand whether OS-01 actually helps our skin function like younger skin, our R&D team measured its effects with MolClock, the first molecular clock capable of measuring skin’s biological age using DNA markers of skin aging.In our test, the biological age of an ex vivo human skin sample started at 70.83 years old. Astoundingly, after only 5 days of exposure to the OS-01 peptide, the skin’s age decreased to just 68.23 years, meaning that the OS-01 peptide reduced the skin’s biological age by 2.5 years in just 5 days! [1]
05

OS-01 Reduces Skin’s Biological Age

With time, our DNA actually changes: accumulating telltale markers of aging. No matter how young your skin looks, these DNA modifications predict whether your skin is functioning like younger, healthier skin or like older, more vulnerable skin.To understand whether OS-01 actually helps our skin function like younger skin, our R&D team measured its effects with MolClock, the first molecular clock capable of measuring skin’s biological age using DNA markers of skin aging.In our test, the biological age of an ex vivo human skin sample started at 70.83 years old. Astoundingly, after only 5 days of exposure to the OS-01 peptide, the skin’s age decreased to just 68.23 years, meaning that the OS-01 peptide reduced the skin’s biological age by 2.5 years in just 5 days! [1]
The biological age of skin was measured by analyzing methylation of human skin samples with MolClock, the first skin-specific molecular clock used to predcit the biological age of skin. [1][2]
These results are perhaps the most exciting of all as they prove that exposure to the OS-01 peptide is capable of reliably reducing the biological age of skin. This means that OS-01 doesn’t just make skin look younger, it also helps skin feel like its younger self.
The biological age of skin was measured by analyzing methylation of human skin samples with MolClock, the first skin-specific molecular clock used to predcit the biological age of skin. [1][2]
These results are perhaps the most exciting of all as they prove that exposure to the OS-01 peptide is capable of reliably reducing the biological age of skin. This means that OS-01 doesn’t just make skin look younger, it also helps skin feel like its younger self.
06

OS-01 Improves Elasticity, Radiance, Firmness, and Wrinkles

At the cellular level, it’s clear that the OS-01 peptide reverses signs of skin aging and restores skin health. But how do these effects show up visibly?The next step was to take the OS-01 peptide out of the lab and put it to the test on real people. For this, we relied on a third-party clinical research organization to evaluate the efficacy of OS-01 FACE, OneSkin’s daily facial topical supplement powered by the OS-01 peptide.
06

OS-01 Improves Elasticity, Radiance, Firmness, and Wrinkles

At the cellular level, it’s clear that the OS-01 peptide reverses signs of skin aging and restores skin health. But how do these effects show up visibly?The next step was to take the OS-01 peptide out of the lab and put it to the test on real people. For this, we relied on a third-party clinical research organization to evaluate the efficacy of OS-01 FACE, OneSkin’s daily facial topical supplement powered by the OS-01 peptide.
The study was performed by a third party research organization and outcomes were evaluated through expert clinical grade, instrumental analysis and consumer perception. n=22, average age= 5 9.5 y. Instrumental evaluation - *vapometer analysis ** Double blind expert clinical grader evaluation
The study was performed by a third party research organization and outcomes were evaluated through expert clinical grade, instrumental analysis and consumer perception. n=22, average age= 5 9.5 y. Instrumental evaluation - *vapometer analysis ** Double blind expert clinical grader evaluation

In a 12-week clinical study, 22 participants with an average age of 59.5 years experienced a significant improvement in skin elasticity and barrier function (+15%) from using OS-01 FACE, correlating with the epidermal thickening we observed in the lab.Additionally, study participants observed:
  • Fewer visible wrinkles & fine lines
  • Firmer, smoother skin
  • Improved radiance & fewer visible pores
  • Better overall skin appearance
By restoring skin health at the cellular level, OS-01 FACE provides noticeable changes to the surface level in just 12 weeks: visibly rejuvenating mature skin.

In a 12-week clinical study, 22 participants with an average age of 59.5 years experienced a significant improvement in skin elasticity and barrier function (+15%) from using OS-01 FACE, correlating with the epidermal thickening we observed in the lab.Additionally, study participants observed:
  • Fewer visible wrinkles & fine lines
  • Firmer, smoother skin
  • Improved radiance & fewer visible pores
  • Better overall skin appearance
By restoring skin health at the cellular level, OS-01 FACE provides noticeable changes to the surface level in just 12 weeks: visibly rejuvenating mature skin.
07

Putting it all together

With the OS-01 peptide, there’s no need for 10-step routines or dozens of supposedly effective ingredients. Just one powerful molecule can help restore and rejuvenate your skin health. All you need is a convenient way to reap its benefits. That’s why we created our topical supplements: transformative longevity treatments in the form of easy-to-use daily moisturizers for the face and body. Pair them with PREP to cleanse your skin every night and reduce your skin routine to just three steps. With our topical supplements, finding the right regimen for mature skin just became so much easier.
07

Putting it all together

With the OS-01 peptide, there’s no need for 10-step routines or dozens of supposedly effective ingredients. Just one powerful molecule can help restore and rejuvenate your skin health. All you need is a convenient way to reap its benefits. That’s why we created our topical supplements: transformative longevity treatments in the form of easy-to-use daily moisturizers for the face and body. Pair them with PREP to cleanse your skin every night and reduce your skin routine to just three steps. With our topical supplements, finding the right regimen for mature skin just became so much easier.

Related Products

Key Takeaways:
  • OneSkin’s proprietary peptide, OS-01, is the first peptide proven to reduce skin’s biological age [1], making it a breakthrough treatment for mature skin.
  • The OS-01 peptide reduces senescent burden in skin [1]–one of the key cellular markers of skin aging.
  • The OS-01 peptide increases epidermal thickness [1], promoting skin strength..
  • By strengthening the skin barrier, the OS-01 peptide can help keep your body protected,providing a potential improvement in overall health.
  • With our topical supplements, you don’t need a 10-step skincare routine. Just daily moisturizers to boost the health of your mature skin.
Key Takeaways:
  • OneSkin’s proprietary peptide, OS-01, is the first peptide proven to reduce skin’s biological age [1], making it a breakthrough treatment for mature skin.
  • The OS-01 peptide reduces senescent burden in skin [1]–one of the key cellular markers of skin aging.
  • The OS-01 peptide increases epidermal thickness [1], promoting skin strength..
  • By strengthening the skin barrier, the OS-01 peptide can help keep your body protected,providing a potential improvement in overall health.
  • With our topical supplements, you don’t need a 10-step skincare routine. Just daily moisturizers to boost the health of your mature skin.

Reviewed by Alessandra Zonari, PhD, Chief Scientific Officer (CSO) and Co-Founder of OneSkin

Alessandra earned her Master’s degree in stem cell biology, and her PhD in skin regeneration and tissue engineering at the Federal University of Minas Gerais in Brazil in collaboration with the 3B’s Research Group in Portugal. Alessandra did a second post-doctoral at the University of Coimbra in Portugal. She is a co-inventor of three patents and has published 20 peer-reviewed papers in scientific journals.

Reviewed by Alessandra Zonari, PhD, Chief Scientific Officer (CSO) and Co-Founder of OneSkin

Alessandra earned her Master’s degree in stem cell biology, and her PhD in skin regeneration and tissue engineering at the Federal University of Minas Gerais in Brazil in collaboration with the 3B’s Research Group in Portugal. Alessandra did a second post-doctoral at the University of Coimbra in Portugal. She is a co-inventor of three patents and has published 20 peer-reviewed papers in scientific journals.

Discover More

Why Is My Neck Aging So Fast?
6 Tips and Tricks for a Youthful Neck
Woman applying sunscreen
Addressing the Need for Indoor Sun Protection
Sunburned skin
The Science of Sunburns: How to Treat and Prevent ...
Get to Know OneSkin’s Topical Supplements:
OS-01 F...
Pollution
Can Air Quality Affect Skin? Exploring the Impact ...
Close up of eye
Understanding the Relationship Between Sleep and U...
Woman not sleeping in bed
Exploring the Effects of Quality Sleep on Your Ski...
Gut microbiom illustration
How to Improve Gut Microbiome
Woman holding bowl of food
Exploring Caloric Restriction and Its Effects on A...
woman stretching in bed
What’s the Connection Between Quality Sleep and Lo...
Healthy foods
Unlocking the Skin Benefits of Intermittent Fastin...
woman exercising
Why Exercise is a Game-Changer for Longevity
The Microbiome Diet and Its Effects on Skin
The Microbiome Diet and Its Effects on Skin
cold water
Cold Exposure: Hype or Longevity Boosting?
healthy foods
Eat Better, Live Longer: Top Foods for Longevity
How Environmental Stressors Affect Skin
How to Protect Your Skin from Everyday Environment...
OS-01 BODY
Pressing the Pause Button on Aging Skin: OS-01 BOD...
lip treatment
Lip Balm Ingredients: What to Look for and What to...
earth
Exploring Blue Zones: How Can We Optimize Our Heal...
Sunburn
Sun Exposure and Your Skin: 6 Things You Need to K...
Lips
Nurturing Your Lips: How to Keep Them Moisturized ...
Lips
Uncovering the 9 Causes of Dry Lips: Why Do Lips G...
How to Travel with Skin Care
How to Travel with Skin Care Products
SHIELD on arm
What is The Difference Between UVA and UVB Rays?
SHIELD on arm
The Importance of Sunscreen: Why Should You Wear I...
Lip sunscreen
Lip Sunscreen: Do Lips Need SPF Protection?
heart hands
The Science of Gratitude
prep on shoulder
Removing Sunscreen: Tips for Clean and Healthy Ski...
freckled skin
Skin Explained: Is Skin An Organ?
UV Light and Skin
UV Light and Skin: Effects and Protection Strategi...
What is Epidermis
Understanding The Epidermis: Functions, Compositio...
Diving into dermis
Diving into Dermis: Functions and Significance
Why Do We Get Wrinkles
Unlocking the Science of Wrinkles: Causes, Formati...
bentonite clay
Ingredient Spotlight: Bentonite Uses, Benefits, & ...
Back skin
Why Skin Barrier is Important
hypodermis function
Hypodermis: Exploring Its Vital Functions
oil-dropper-background-pink-dripping-cosmetic-product
The EU is Limiting Retinol in Skincare: Here’s Eve...
face+shield
Why You Should Use OS-01 FACE & OS-01 SHIELD Toget...
Age Gracefully: How to Manage Neck Wrinkles and Ac...
Discover Why Our New SPF Outperforms the Rest
How to Preserve Your Feet
How to Preserve Your Feet: 5 Strategies for Wrinkl...
Hands and Dermal Elasticity: Understanding and Man...
The Lip Lineage
The Lip Lineage: Exploring the Why Behind Wrinkled...
A Journey Through the Skin: How UV Radiation Cause...
How to Address Stomach Wrinkles
How to Address Stomach Wrinkles For Smoother Skin
How to Get Rid of Wrinkly Skin on Arms
A Scientific Approach to Addressing Wrinkly Skin o...
What is Skin Cycling
What is Skin Cycling? Understanding, Embracing, an...
How to Skin Cycle: A Guide to Optimize Your Skin C...
How Do Our Products Work Together?
Do You Know What Clinical Claims Really Mean?
What is Tinted Sunscreen?
What is Tinted Sunscreen?
two types of face wrinkles
2 Types of Wrinkles and Tips for Treating Them
 Zinc Oxide
The Top 5 Benefits of Zinc Oxide for Skin
Marionette Lines
Understanding Marionette Lines: Causes and Prevent...
How to Help Your Skin Act Younger
Better Elasticity, More Firmness: Discover OS-01 E...
This is the Data to Look For When Choosing Skin Ca...
Better Elasticity, More Firmness: Discover OS-01 E...
This is the Data to Look For When Choosing Skin Ca...
how-much-sunscreen-to-use-on-face
The Science of Sunscreen: Understanding the Optima...
Symptoms and Causes of Melasma on Face
Benefits of Sunscreen
Beyond UV Protection: 7 Surprising Ways Sunscreen ...
Mineral vs Chemical Sunscreen
Making Sense of Sunscreen: Understanding the Diffe...
Navigating Anti-Aging Skincare: The industry, the ...
Sun spots
Sun Spots: Causes & 3 Prevention Tips
sunscreen-ingredients-to-avoid
Navigating Sunscreen Labels: Unwanted Ingredients ...
How to Prevent Skin Aging Before It Starts Moved
How to Prevent Skin Aging Before It Starts
Face vs. Body Sunscreen: Can You Use Body Sunscreen On Your Face?
Face vs. Body Sunscreen: Can You Use Body Sunscree...
Sun Damaged Skin: What it is, how you can prevent ...
Thick Skin vs Thin Skin
A Comprehensive Guide to Understanding the Differe...
Thick Skin vs Thin Skin
A Comprehensive Guide to Understanding the Differe...
How to Strengthen Your Skin
How to Strengthen Your Skin: A Guide to Thicker, H...
How to Strengthen Your Skin
How to Strengthen Your Skin: A Guide to Thicker, H...
Difference Between Hydrating and Moisturizing
Exploring the Difference Between Hydrating and Moi...
Difference Between Hydrating and Moisturizing
Exploring the Difference Between Hydrating and Moi...