Scientific Breakthrough: OneSkin’s scientists show that it’s possible to reverse aging in human skin
6 min read
JUNE 1, 2023
Scientific Breakthrough: OneSkin’s scientists show that it’s possible to reverse aging in human skin
6 min read
JUNE 1, 2023
After over 7 years of research, our team recently achieved an important milestone: getting our data published in npj Aging. This is significant not just for OneSkin, but for the longevity community at large, because it shows for the first time that it is possible to reverse aging in human skin. Core to this finding is our evidence that the OS-01 peptide is able to reduce biological age and senescence burden in human skin models. Because of its proven ability to block the effects of senescence while enhancing cellular renewal, OS-01 is now validated as a senotherapeutic molecule, making its development an exciting new breakthrough in the quest to promote human longevity. Curious to explore the data in our paper? We’ve summarized its key findings below.
After over 7 years of research, our team recently achieved an important milestone: getting our data published in npj Aging. This is significant not just for OneSkin, but for the longevity community at large, because it shows for the first time that it is possible to reverse aging in human skin. Core to this finding is our evidence that the OS-01 peptide is able to reduce biological age and senescence burden in human skin models. Because of its proven ability to block the effects of senescence while enhancing cellular renewal, OS-01 is now validated as a senotherapeutic molecule, making its development an exciting new breakthrough in the quest to promote human longevity. Curious to explore the data in our paper? We’ve summarized its key findings below.
01
Finding 1: The OS-01 Peptide Reverses Skin’s Biological Age
Utilizing MolClock, OneSkin’s proprietary, peer-reviewed, skin-specific DNA methylation clock, we measured the biological age of ex vivo skin samples before and after exposure to the OS-01 peptide.
01
Finding 1: The OS-01 Peptide Reverses Skin’s Biological Age
Utilizing MolClock, OneSkin’s proprietary, peer-reviewed, skin-specific DNA methylation clock, we measured the biological age of ex vivo skin samples before and after exposure to the OS-01 peptide.
DNA methylation age of ex vivo human skin samples (79yr) treated with 12.5uM peptide for 5 days, calculated using OneSkin’s Skin-Specific DNA Methylation Clock, MolClok. OS-01 peptide treatment significantly reduced the DNA methylation age (p<0.01). (Zonari, et al)
Before exposure, the biological age of the skin samples measured at 70.82 years old. After exposure to the OS-01 peptide for 5 days, the biological age of the same samples measured at 68.22 years, meaning that the OS-01 peptide reduced the biological age of skin by 2.6 years in ex vivo human skin samples (Zonari, et al). Besides MolClock, a reduction in the biological age of ex vivo skin treated with the OS-01 peptide was also detected using other published molecular clocks, including Horvarth Clock, Horvath Skin & Blood Clock, Hannum Clock, Lin Clock, and PhenoAge Clock, further confirming the age reversal capability of the OS-01 peptide (Zonari, et al).
DNA methylation age of ex vivo human skin samples (79yr) treated with 12.5uM peptide for 5 days, calculated using OneSkin’s Skin-Specific DNA Methylation Clock, MolClok. OS-01 peptide treatment significantly reduced the DNA methylation age (p<0.01). (Zonari, et al)
Before exposure, the biological age of the skin samples measured at 70.82 years old. After exposure to the OS-01 peptide for 5 days, the biological age of the same samples measured at 68.22 years, meaning that the OS-01 peptide reduced the biological age of skin by 2.6 years in ex vivo human skin samples (Zonari, et al). Besides MolClock, a reduction in the biological age of ex vivo skin treated with the OS-01 peptide was also detected using other published molecular clocks, including Horvarth Clock, Horvath Skin & Blood Clock, Hannum Clock, Lin Clock, and PhenoAge Clock, further confirming the age reversal capability of the OS-01 peptide (Zonari, et al).
02
Finding 2: The OS-01 Peptide Promotes a Reduction in Senescent Cells
To confirm their findings, our scientists took a look at how OS-01 impacted levels of cellular senescence, a central hallmark of aging that largely drives the skin’s biological age.Using various senescence-inducing experimental models to reproduce both intrinsic (e.g. chronologically aged) and extrinsic (e.g. UVB radiation) stimuli, our scientists looked at whether OS-01 could reduce the total number of senescent cells. (Zonari, et al)
02
Finding 2: The OS-01 Peptide Promotes a Reduction in Senescent Cells
To confirm their findings, our scientists took a look at how OS-01 impacted levels of cellular senescence, a central hallmark of aging that largely drives the skin’s biological age.Using various senescence-inducing experimental models to reproduce both intrinsic (e.g. chronologically aged) and extrinsic (e.g. UVB radiation) stimuli, our scientists looked at whether OS-01 could reduce the total number of senescent cells. (Zonari, et al)
SA B-Gal (senescence-associated beta galactosidase) stained image of in vitro HGPS HDFs (human dermal fibroblasts from patient-derived samples with Hutchinson-Gilford progeria syndrome) treated with 50uM OS-01 peptide for 2 days compared to untreated control (Zonari, et al).For their intrinsic aging experiments, our researchers obtained skin samples from patients with Hutchinson-Gilford progeria syndrome, a genetic condition that causes premature skin aging with a high level of cellular senescence. Treating in vitro fibroblast cultures from these patients, our team applied senescence-associated beta galactosidase, a compound that selectively stains senescent cells blue. This made it possible to count the number of senescent cells before and after exposure to the OS-01 peptide.After 2 days of treatment, our scientists found that the samples treated with the OS-01 peptide contained up to 50% fewer senescent cells than samples that were not treated. Additionally, the total number of cells remained the same after exposure to the OS-01 peptide, showing that the OS-01 peptide promoted not just the reduction of senescent cells, but also the renewal of healthier ones. (Zonari, et al)Across many experimental models, the OS-01 peptide decreased the level of cellular senescence, promoting tissue rejuvenation in both 2D and 3D systems, as measured by cellular and molecular markers of aging, senescence, and DNA methylation .(Zonari, et al)Many skin care brands claim rejuvenation and reversing aging, but OneSkin is the only brand proven to actually do it. We hope the publication of our research in npj Aging will serve as a model for how evidence-based science can meet clinical efficacy in the skin health industry. Want to read more about our research? You can find our full published paper Senotherapeutic peptide treatment reduces biological age and senescence burden in human skin models, hereto learn more about nature.
SA B-Gal (senescence-associated beta galactosidase) stained image of in vitro HGPS HDFs (human dermal fibroblasts from patient-derived samples with Hutchinson-Gilford progeria syndrome) treated with 50uM OS-01 peptide for 2 days compared to untreated control (Zonari, et al).For their intrinsic aging experiments, our researchers obtained skin samples from patients with Hutchinson-Gilford progeria syndrome, a genetic condition that causes premature skin aging with a high level of cellular senescence. Treating in vitro fibroblast cultures from these patients, our team applied senescence-associated beta galactosidase, a compound that selectively stains senescent cells blue. This made it possible to count the number of senescent cells before and after exposure to the OS-01 peptide.After 2 days of treatment, our scientists found that the samples treated with the OS-01 peptide contained up to 50% fewer senescent cells than samples that were not treated. Additionally, the total number of cells remained the same after exposure to the OS-01 peptide, showing that the OS-01 peptide promoted not just the reduction of senescent cells, but also the renewal of healthier ones. (Zonari, et al)Across many experimental models, the OS-01 peptide decreased the level of cellular senescence, promoting tissue rejuvenation in both 2D and 3D systems, as measured by cellular and molecular markers of aging, senescence, and DNA methylation .(Zonari, et al)Many skin care brands claim rejuvenation and reversing aging, but OneSkin is the only brand proven to actually do it. We hope the publication of our research in npj Aging will serve as a model for how evidence-based science can meet clinical efficacy in the skin health industry. Want to read more about our research? You can find our full published paper Senotherapeutic peptide treatment reduces biological age and senescence burden in human skin models, hereto learn more about nature.
Key Takeaways:
- The OneSkin team recently published key findings on the OS-01 peptide in npj Aging, a highly reputable scientific journal.
- For the first time, OneSkin’s scientists show that it’s possible to reverse aging in human skin
- Not only does our publication validate our research, it's also a huge milestone in advancing the science of aging
- The research published shows that the OS-01 peptide diminishes skin’s biological age by decreasing levels of cellular senescence.
- Because of its proven ability to block the effects of senescence while enhancing cellular renewal, OS-01 is an exciting breakthrough in the quest to treat aging and extend human lifespan.
Key Takeaways:
- The OneSkin team recently published key findings on the OS-01 peptide in npj Aging, a highly reputable scientific journal.
- For the first time, OneSkin’s scientists show that it’s possible to reverse aging in human skin
- Not only does our publication validate our research, it's also a huge milestone in advancing the science of aging
- The research published shows that the OS-01 peptide diminishes skin’s biological age by decreasing levels of cellular senescence.
- Because of its proven ability to block the effects of senescence while enhancing cellular renewal, OS-01 is an exciting breakthrough in the quest to treat aging and extend human lifespan.
Reviewed by Alessandra Zonari, PhD, Chief Scientific Officer (CSO) and Co-Founder of OneSkin
Alessandra earned her Master’s degree in stem cell biology, and her PhD in skin regeneration and tissue engineering at the Federal University of Minas Gerais in Brazil in collaboration with the 3B’s Research Group in Portugal. Alessandra did a second post-doctoral at the University of Coimbra in Portugal. She is a co-inventor of three patents and has published 20 peer-reviewed papers in scientific journals.
Reviewed by Alessandra Zonari, PhD, Chief Scientific Officer (CSO) and Co-Founder of OneSkin
Alessandra earned her Master’s degree in stem cell biology, and her PhD in skin regeneration and tissue engineering at the Federal University of Minas Gerais in Brazil in collaboration with the 3B’s Research Group in Portugal. Alessandra did a second post-doctoral at the University of Coimbra in Portugal. She is a co-inventor of three patents and has published 20 peer-reviewed papers in scientific journals.
Discover More
A Comprehensive Guide to Understanding and Managin...
What Is Causing the Uneven Skin Tone On Your Body?
Celebrating Black History Month: A Discussion With...
Skin Hyperpigmentation 101: What It Is & How To Tr...
Good Genes vs. Good Habits: Which Impacts Your Lon...
How Do Our Products Work Together?
How to Reduce Skin Inflammation & Redness
How to Strengthen Your Skin: A Guide to Thicker, H...
Better Elasticity, More Firmness: Discover OS-01 E...
The Top 4 Prickly Pear Benefits for Skin
How To Get Rid of Crepey Skin: 7 Solutions
Moved - A Comprehensive Guide to Understanding and...
Why cellular senescence is more than just one of t...
How OS-01 Works on Mature Skin
7 Causes For Crepey Skin Under Eyes
What Does Hyaluronic Acid Do For Your Skin?
Longevity Lifestyle #4 - Sleep Optimization
When to Apply Sunscreen: Before or After Moisturiz...
This is the Data to Look For When Choosing Skin Ca...
Senotherapeutics, Senolytics, and Senomorphics -- ...
This is the Data to Look For When Choosing Skin Ca...
How is OS-01 EYE different from OS-01 FACE?
Biological Age: What is Biological Age, Why Does i...
How to Incorporate OneSkin’s OS-01 Topical Supplem...
Exploring the Difference Between Hydrating and Moi...
How OS-01 Can Help Your Collagen Levels As You Age
Retinol 101: Debunking Retinol as the Gold Standar...
An In-Depth Look at Glycerin: What is it and What ...
Peptides vs Retinol: Is One Better For Increasing ...
Moved - An In-Depth Look At The Benefits Of Moring...
Is Fragrance Bad for Your Skin?
Cell Turnover & Why It Slows As We Age
What your skincare routine is missing (Hint. It’s ...
OneSkin launches MolClock, the first skin-specific...
Is This The Root Cause of Aging?
The Top 10 Best Foods for Skin Repair
The 101 Guide To Understanding Acne-Prone Skin
Three Ways To Extend Your Skinspan Starting With Y...
UV Damage Increases Cellular Senescence. Here's Ho...
What is Skin Cycling? Understanding, Embracing, an...
Moved - How to Tighten the Skin Under Your Eyes
How To Boost Skin Moisture For Your Entire Body Fr...
Calorie Restriction And Intermittent Fasting: Eati...
Gut Health and Skin: How Are They Connected?
How Aging Science Will Help Us Fight Future Pandem...
What Foods Cause Acne and Which Fight Against It?
4 Steps to Crafting the Perfect Skin Care Routine ...
How to Prevent Skin Aging Before It Starts
3 Natural Ways to Keep Your Skin Hydrated and Glow...
4 Essential Face Cleansing Steps to Achieving a Ra...
A Simplified Guide To The Different Skin Texture T...
How to Brighten Eyes and Under-Eyes
Yes, You Need Sunscreen During Winter
Moved - UV Damage Increases Cellular Senescence. H...
Navigating Anti-Aging Skincare: The industry, the ...
Clean Beauty: What “clean” really means and why “s...
101 Guide on The Benefits of Cleansing Your Face
Get to Know OneSkin’s Topical Supplements:
...OS-
Invasive vs. Non-Invasive Skincare: The pros and c...
4 Foods To Avoid During Wound Healing & Why
Do You Know What Clinical Claims Really Mean?
Making Sense of Sunscreen: Understanding the Diffe...
Symptoms and Causes of Melasma on Face
2 Types of Wrinkles and Tips for Treating Them
Are There Dangers of Retinol Cream Use
Discover Why Our New SPF Outperforms the Rest
Navigating Sunscreen Labels: Unwanted Ingredients ...
Beyond UV Protection: 7 Surprising Ways Sunscreen ...
The Science of Sunscreen: Understanding the Optima...
Understanding Marionette Lines: Causes and Prevent...
Hands and Dermal Elasticity: Understanding and Man...
The Top 5 Benefits of Zinc Oxide for Skin
What is Epidermal Thickness & Why Does it Matter?
Winter Skincare - How To Prevent Dry Skin this Sea...
Can a Plant-Based Diet Really Increase Your Health...
How to Know if Your Skin Barrier is Damaged
Tight Feeling Skin: 3 Reasons Why
Our Story: Learn more about the history of OneSkin...
How to Strengthen Your Skin: A Guide to Thicker, H...
What is the Function of Skin as a Protective Barri...
Can You Use Hyaluronic Acid with Retinol?
OneSkin Featured In Tony Robbins’ Life Force
A Comprehensive Guide to Understanding the Differe...
Stressed Skin: 4 Stress Effects on the Skin
How Relationships, Community, & Social Interaction...
How OneSkin Developed the OS-01 Peptide
Better Elasticity, More Firmness: Discover OS-01 E...
Exploring the skin’s purpose in whole-body health
What Are The Causes Of Dry, Flaky Skin On Your Fac...
Senotherapeutics, Senolytics, and Senomorphics -- ...
Dry Skin 101: How To Identify This Skin Type Moved
Are Parabens Bad for Your Skin?
How To Smooth Skin Texture On The Face: 4 Expert T...
What is Tinted Sunscreen?
Face vs. Body Sunscreen: Can You Use Body Sunscree...
Sun Spots: Causes & 3 Prevention Tips
What Are Free Radicals and How Do They Impact Skin...
Our Research Has Been Published in npj Aging
6 Factors That Contribute to Slow Skin Healing
How To Create A Hydrating Skin Care Routine For Dr...
More Than Skin Deep: How Physical Touch Predicts L...
What is a peptide?
Peptides: the first piece o
...Peptides: the first piece o
What happens to skin as a person ages?
How to Address Stomach Wrinkles For Smoother Skin
Target Cellular Senescence with the Highest Concen...
The Science of Gratitude
101 Guide To Restoring Dry Skin Around The Eyes
How To Reduce Hyperpigmentation by Targeting Cellu...
How to Prevent Skin Aging Before It Starts Moved
The Science Behind Why The Skin Around the Eyes Ag...
The Top 6 Best Antioxidants For Skin Health and Lo...
Hallmarks Of Aging
4 Essential Face Cleansing Steps to Achieving a Ra...
What is Sustainable Packaging for Cosmetic Product...
SKIN CARE IS HEALTH CARE: WHY EXTENDING YOUR SKINS...
What is Skin Inflammation? What Causes it?
Safe Beauty, Validated: Why OneSkin trusts SkinSAF...
101 Guide To Restoring Dry Skin Around The Eyes Mo...
How Do You Know When to Stop Using Retinol?
A Step-by-Step Guide to Creating the Perfect Skin ...
Special Announcement: OneSkin to Donate 1% of Tota...
Cleanser Vs. Face Wash: Which Should I Reach For?
Retinol 101: Debunking Retinol as the Gold Standar...
Health Is The New Wealth
Young skin - how to keep it not just looking young...
How to Reverse (or Prevent) Aging Skin
What is Normal Skin & Do I Have This Skin Type?
Understanding Oily Skin: Causes & Characteristics
OneSkin’s 12-Week Clinical Study to Validate the E...
How chronic stress makes you age faster
How OS-01 EYE Supports the Ultra-Thin Skin Around ...
Eye Skin Ages Faster: Here’s How OS-01 Can Help
7 Causes For Crepey Skin Under Eyes Moved
7 Benefits of Andiroba Oil For Skin
How to Use Peptides In Your Skincare Regimen to Op...
An In-Depth Look at Glycerin: What is it and What ...
What is Sensitive Skin? Moved
What Causes Dark Circles Under and Around Your Eye...
What Causes Crepey Skin & Can It Be Avoided Altoge...
Exploring the Difference Between Hydrating and Moi...
How To Create A Hydrating Skin Care Routine For Dr...
Does Niacinamide Help With Acne & Scarring?
How to Repair & Restore Your Skin Barrier
Moved - A Step-by-Step Guide to Creating the Perfe...
OneSkin Notable Mention: Dave Asprey's The Human U...
The Lip Lineage: Exploring the Why Behind Wrinkled...
How OS-01 Works With Different Skin Types
What Is Exfoliation & Is It A Necessary Measure Fo...
What Do Antioxidants Do For Skin and Why Should Yo...
Exploring the Benefits of Glycerin for Skin Health...
How Does Sunscreen Work? How Long Does it Last?
A Journey Through the Skin: How UV Radiation Cause...
Skin Microbiome: What It Is, Why it’s Important, A...
Why We Want Skin To Be More, Not Less